Which of the Following Describes the Most Likely Effect That

Multiple Choice

Which of the following describes the most likely effect that a single base substitution in the middle of gene's sequence will have on the protein the gene encodes?

A) A single amino acid would change because pairing of mRNA and tRNA in the ribosome would change.
B) The protein would be shorter because the correct tRNA would not be able to interact with the ribosome.
C) All amino acids following the substitution would be affected because the reading frame would be altered.
D) There would be no change because the genetic code is ambiguous.