Rank the Following Mutations with Respect to Their Likelihood of Affecting

Multiple Choice

Rank the following mutations with respect to their likelihood of affecting the structure of the polypeptide (from most likely to affect structure to least likely) .
1) insertion mutation in the middle of an intron
2) deletion of 3 bases crossing an intron-exon border
3) substitution mutation at the third position of a codon
4) substitution mutation at the second position of a codon

A) 1, 2, 4, 3
B) 2, 3, 4, 1
C) 3, 4, 2, 1
D) 4, 3, 2, 1