You Performed a Co-Immunoprecipitation Experiment to Identify Cellular Proteins Associated

Short Answer

You performed a co-immunoprecipitation experiment to identify cellular proteins associated with protein X. For the experiment, you used antibodies against protein X that were immobilized on beads. You incubated these beads with cell lysates from either wild-type cells or cells lacking the gene encoding protein Y. After washing and eluting the bound protein off the beads, you separated each bound and unbound fraction by SDS-PAGE. Finally, you performed immunoblotting using antibodies against either protein Y or protein Z. The results of the Western blots are presented in the following schematic drawings. According to these results, do you think protein Y enhances (E) or inhibits (I) binding of protein X to protein Z? Write down E or I as your answer.