Considering the Following Hydropathy Plot for a Multipass Transmembrane Protein

Multiple Choice

Considering the following hydropathy plot for a multipass transmembrane protein in the plasma membrane, are the protein termini expected to be located inside (I) or outside (O) of the cell? Which of the regions indicated as 2 and 3 in the plot is expected to have more positively charged residues? The position of positively charged residues near the first transmembrane helix is indicated by an asterisk on the plot.

A) I; 2
B) I; 3
C) O; 2
D) O; 3