How Is the Reference Rage of an Assay Determined

Multiple Choice

How is the reference rage of an assay determined?

A) By testing 100-150 known standards and determining the range that runs from the lowest value to the highest value
B) By testing 100-150 specimens that represent the laboratory's patient population and determining the range that runs from 2 SD below the mean to 2 SD above the mean
C) By testing 100-150 specimens that represent the laboratory's patient population and ordering the values from lowest to highest with the lowest and highest values representing the range
D) None of the above