Exhibit 13A This Is a Map of the PBR322 Plasmid

Multiple Choice

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. Refer to Exhibit 13A. If a recombinant plasmid were obtained by inserting DNA into the Eco RV site, and the protein corresponding to the recombinant gene were expressed, which of the following statements would be false ?

A) The protein will probably contain some extra amino acids on its N-terminal.
B) The plasmid will be resistant to ampicillin.
C) The plasmid will not be able to replicate autonomously.
D) The protein is not likely to be biologically active without some further treatment.
E) The question cannot be answered, since it is unlikely that the protein will be expressed at all, because there is no promoter for mRNA synthesis.