Sometimes DNA Probes Are Used to Prove That a Gene

Multiple Choice

Sometimes DNA probes are used to prove that a gene has been incorporated into a eukaryotic genome. The following are all steps used in such a procedure, except :

A) Digestion of the cellular DNA to break it into manageable sizes.
B) Separation of the fragments by gel electrophoresis.
C) Hybridization of a nucleic acid strand complementary to the gene of interest.
D) Elution of the hybridized DNA from the gel for analysis.
E) All of these steps are necessary in probing for the presence of a gene.