Which of the Following Was Not Evidence That Supported Mitchell's

Multiple Choice

Which of the following was not evidence that supported Mitchell's chemiosmotic coupling hypothesis?

A) Closed vessicles were required. Phosphorylation did not occur in a completely soluble environment.
B) Vessicles could be prepared from mitochondria and the assymetric location of electron transport protein could be shown.
C) The existence of the pH gradient could be demonstrated
D) The transfer of electrons from complex I to oxygen was shown
E) none of these