How Do Scientists Determine the Annealing Temperature Used in PCR

Multiple Choice

How do scientists determine the annealing temperature used in PCR reactions? ​

A) It is always 55ᵒC because the DNA polymerase works best at this temperature.
B) It is always 55ᵒC because primers anneal best at lower temperatures.
C) It is always 60ᵒC because any other temperature would destabilize the DNA.
D) It can vary from 55-60ᵒC depending on the primers being used.
E) It can vary from 55-60ᵒC depending on the type of DNA polymerase being used.