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Match the Steps of Two Rounds of PCR with an Appropriate

Question 22

Matching

Match the steps of two rounds of PCR with an appropriate description.

Premises:

Responses:

DNA with a targeted sequence is mixed with primers, nucleotides, and Taq DNA polymerase.

The mixture is heated, and the double-stranded DNA separates into single strands. When the mixture is cooled, some of the primers base-pair with the DNA at opposite ends of the targeted sequence.

Taq polymerase begins DNA synthesis at the primers, so it produces complementary strands of the targeted DNA sequence.

All double-stranded DNA separates into single strands after mixture is reheated. After it cools, primers base-pair with the targeted sequence in the original template DNA and in the new DNA strands.

The number of copies of the targeted DNA section is doubled with each cycle of heating and cooling. After two rounds, the amount of the initial DNA template has doubled twice.

Premises:

DNA with a targeted sequence is mixed with primers, nucleotides, and Taq DNA polymerase.

The mixture is heated, and the double-stranded DNA separates into single strands. When the mixture is cooled, some of the primers base-pair with the DNA at opposite ends of the targeted sequence.

Taq polymerase begins DNA synthesis at the primers, so it produces complementary strands of the targeted DNA sequence.

All double-stranded DNA separates into single strands after mixture is reheated. After it cools, primers base-pair with the targeted sequence in the original template DNA and in the new DNA strands.

The number of copies of the targeted DNA section is doubled with each cycle of heating and cooling. After two rounds, the amount of the initial DNA template has doubled twice.

Responses:

Correct Answer:

Premises:

Responses:

DNA with a targeted sequence is mixed with primers, nucleotides, and Taq DNA polymerase.

The mixture is heated, and the double-stranded DNA separates into single strands. When the mixture is cooled, some of the primers base-pair with the DNA at opposite ends of the targeted sequence.

Taq polymerase begins DNA synthesis at the primers, so it produces complementary strands of the targeted DNA sequence.

All double-stranded DNA separates into single strands after mixture is reheated. After it cools, primers base-pair with the targeted sequence in the original template DNA and in the new DNA strands.

The number of copies of the targeted DNA section is doubled with each cycle of heating and cooling. After two rounds, the amount of the initial DNA template has doubled twice.