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A Plasmid Cloning Vector and DNA Fragments Containing the XYZ

Question 78

Multiple Choice

A plasmid cloning vector and DNA fragments containing the XYZ gene, each digested with the same restriction enzyme, are mixed with DNA ligase. Which product(s) will result?


A) Recombinant plasmids only
B) Nonrecombinant plasmids only
C) Joined-together copies of the DNA fragment containing the XYZ gene only
D) A mixture of recombinant plasmids, nonrecombinant plasmids, and joined-together copies of the DNA fragment containing the XYZ gene
E) No reaction will occur because DNA polymerase, rather than DNA ligase, is needed to insert the DNA fragments into the plasmid cloning vector

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