40 Identify the Correct Steps for Cloning a Gene of Interest

Multiple Choice

40. Identify the correct steps for cloning a gene of interest, then arrange them in the correct order. One of the steps below is left out of the answer. 1. Transform the bacteria.
2)  Incubate the DNA fragments and cut cloning vector with DNA ligase.
3)  Use restriction enzymes to cut the gene of interest and the cloning vector.
4)  Spread bacteria on medium containing lactose and ampicillin.
5)  Spread bacteria on medium containing X-gal and ampicillin.

A) 4, 3, 1, 5
B) 3, 2, 5, 1
C) 3, 2, 1, 5
D) 2, 3, 1, 5
E) 3, 2, 1, 4