The Chemical Difference Between Dideoxyribonucleotides Used in Sanger Sequencing and the Deoxyribonucleotides

Multiple Choice

The chemical difference between dideoxyribonucleotides used in Sanger sequencing and the deoxyribonucleotides normally found in DNA is the presence of a(n) _____ in the deoxyribose sugar of dideoxyribonucleotides.

A) 3' - H rather than - OH
B) 5' - H rather than - OH
C) 5' - OH rather than - H
D) 5'  - H rather than - OH
E) 3' - COOH rather than - OH