In the Whole-Genome Shotgun Method, the Genome Is Fragmented and Individual

Multiple Choice

In the whole-genome shotgun method, the genome is fragmented and individual fragments are sequenced. How is the order of the nucleotides determined for the original intact chromosome?

A) The fragmentation is done in a systematic way such that the physical arrangement of fragments is readily apparent.
B) The random fragmentation produces overlapping sequences that can be aligned and assembled to generate the original intact DNA molecule.
C) We supplement our information with data from a different technique to determine the final chromosome arrangement.
D) DNA hybridization assays are conducted to determine the physical arrangement of the genes on the chromosome.
E) After sequencing, the fragments are labeled and used as probes in a microarray.