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Bacteria Are Grown in 15N (Heavy)medium and Then Transferred to 14N

Question 14

Multiple Choice

Bacteria are grown in 15N (heavy) medium and then transferred to 14N (light) medium and are allowed to replicate for 1 generation.The DNA is subsequently isolated and centrifuged in a CsCl2 gradient to yield what type of gradient band(s) ?


A) one heavy band
B) one light band
C) one half-heavy (intermediate to heavy and light) band
D) one heavy and one half-heavy band
E) one heavy and one light band

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