What Is One Major Obstacle That Using the Transcriptome (Single-Cell

Multiple Choice

What is one major obstacle that using the transcriptome (single-cell transcriptomics) would present in classifying neurons?

A) The transcriptome of any cell is dynamic-it changes constantly, depending on the demands of the cell and any point in time, as well as on the type of mRNA being classified (e.g., spliced? processed?)
B) Technology for this practice is still not advanced enough.
C) The RNA-Seq database applies only to neurons of the same overall function, such as all cortical neurons or all hippocampal neurons or all cerebellar neurons.
D) mRNA is too unstable and difficult to work with successfully.
E) This technology is fraught with logistical problems, such as corroborating the same results from different labs.