Which of the Following Tests Would Be Effective at Determining

Multiple Choice

Which of the following tests would be effective at determining if a transmitter action was mediated by a G protein-coupled receptor?

A) Adding extra GTP to the cell cytoplasm to activate G protein-coupled receptors.
B) The addition of cholera toxin to inhibit G protein-coupled receptors.
C) The addition of GDP-β-s enhances and greatly prolongs agonist-induced activation of G protein-mediated responses.
D) The addition of GTP-γ-s enhances and greatly prolongs agonist-induced activation of G protein-mediated responses.
E) The addition of pertussis toxin to activate G protein-coupled receptors.