Design of an Effective ELISA Can Be Challenging Because

Multiple Choice

Design of an effective ELISA can be challenging because

A) different detection systems have sensitivity that ranges over five orders of magnitude.
B) the number of replicates required for any ELISA is quite high.
C) the anticipated concentration of targets must be estimated to determine the sensitivity required of the assay.
D) signal amplification by the assay conditions can impair interpretations.
E) All of the answers are correct.