What Information Is Critical to the Success of Polymerase Chain

Multiple Choice

What information is critical to the success of polymerase chain reaction (PCR) ?

A) The DNA sequence of the ends of the DNA to be amplified must be known.
B) The complete DNA sequence of the DNA to be amplified must be known.
C) The sequence of restriction-enzyme recognition sites in the DNA to be amplified must be known.
D) The sequence of restriction-enzyme recognition sites in the DNA to be amplified and in the
Plasmid where the amplified DNA fragment will be cloned must be known.
E) The plasmid must have a low GC content.