Which of the Following Is in the Correct Order for One

Multiple Choice

Which of the following is in the correct order for one cycle of polymerase chain reaction (PCR) ?

A) Denature DNA; add fresh enzyme; anneal primers; add dNTPs; extend primers.
B) Anneal primers; denature DNA; extend primers.
C) Extend primers; anneal primers; denature DNA.
D) Denature DNA; anneal primers; extend primers.
E) Denature DNA; add fresh enzyme; add dNTPs; anneal primers; extend primers.