Several Techniques Are Available for Carrying Out Amino Acid Analysis

Multiple Choice

Several techniques are available for carrying out amino acid analysis. The amide bonds of proteins and peptides can be hydrolyzed in acidic or basic aqueous solutions, then separated by chromatography and analyzed. Which statement is true?

A) Hydrolyzed amino acids react with AQC-NHS on the carboxylic acid.
B) In C18-HPLC, the amino acids with polar side chains are absorbed more strongly on the stationary phase.
C) Amino acid analysis gives the relative amounts of each amino acid residue.
D) Amino acid analysis gives the order of the amino acid residues.