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Scientists at Cal Tech (Pasadena, Ca

Question 23

Multiple Choice

Scientists at Cal Tech (Pasadena, Ca. and Applied Biosystems (Foster City, Ca.) improved upon the Sanger sequencing method. Which one of the following statements is not true.


A) Replacing the use of radioactive isotopes with fluorescent dyes
B) Detection of these dyes using UV-vis spectrophotometer
C) A machine developed by Applied Biosystems that was an automated sequencing instrument
D) Employed the Sanger approach with ddNTPs as terminating bases
E) Primers were labeled with fluorescent dyes (dye primer approach)

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