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In a Melting Profile for DNA, the Absorbance at 260

Question 26

Multiple Choice

In a melting profile for DNA, the absorbance at 260 nm increases as result of the disruption of the DNA structure. What is the fundamental physical basis for the absorbance increase?


A) the effective concentration of DNA changes
B) denatured DNA absorbs light more strongly than native DNA because the bases have become unstacked
C) native DNA absorbs light more strongly than denatured DNA because the bases are stacked
D) the absorbance of DNA is highly sensitive to temperature

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