A Histotechnician Needs to Re-Stain an Old Slide for Repeat

Multiple Choice

A histotechnician needs to re-stain an old slide for repeat microscopy of the Pathologist. What is the proper order before re-staining and the proper reagents to be used?

A) Immerse the old slide in bleach for 24 hours, remove the cover slip, immerse in Xylene for 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse with 5% oxalic acid for 5 minutes, rinse, and re-stain with the appropriate stain.
B) Immerse the old slide in Xylene for 24 hours, remove the cover slip, immerse in Xylene for another 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse in 5% oxalic acid for 5 minutes, rinse, and re-stain with the appropriate stain.
C) Immerse the old slide in Xylene for 24 hours, remove the cover slip, immerse in Xylene for another 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse in 5% picric acid for 5 minutes, rinse, and re-stain with the appropriate stain.
D) Immerse the old slide in bleach for 24 hours, remove the cover slip, immersed in Xylene for 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse in 5% glacial acetic acid for 5 minutes, rinse, and re-stain with the appropriate stain.