Essay
You have purified a transcription factor that has a leucine-rich region. You perform an electrophoretic mobility shift assay (EMSA) using a double-stranded oligonucleotide that you know from other studies contains the site recognized by this transcription factor in vivo. However, the transcription factor does not bind to the labeled oligonucleotide in your EMSA. Provide an explanation for this result.
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The transcription factor is likely to co...View Answer
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