How Can an Amino Acid Sequence Be Used to Design

Multiple Choice

How can an amino acid sequence be used to design a gene-specific hybridization probe?

A) Synthesize a portion of a polypeptide chain based on the amino acid sequence of the full protein, and use this polypeptide chain as a probe.
B) Purify a protein that contains this amino acid sequence, digest it with proteases that cleave at specific sites, and use one of these peptide fragments as a probe.
C) Synthesize all possible nucleotide sequences long enough to encode the amino acid sequence and thereby find one sequence that will encode the amino acid sequence.
D) Synthesize a set of nucleotide sequences that could encode the amino acid sequence, and this set of oligonucleotides as a probe.