It Is Common to Use DdNTPs (Dideoxynucleoside Triphosphates) for Sequencing

Multiple Choice

It is common to use ddNTPs (dideoxynucleoside triphosphates) for sequencing DNA because, when incorporated into a growing DNA polymer, the ddNTP acts as a chain terminator, allowing the researcher an opportunity to determine the last-added base to a given chain. From your knowledge of nucleotide structure and DNA synthesis, what is the most likely attribute of ddNTPs for DNA sequencing?

A) The ddNTPs lack a nitrogenous base.
B) The ddNTPs lack the sugar component.
C) The ddNTPs lack a 5 ' carbon.
D) The ddNTPs lack a 3 ' hydroxyl group.