Multiple Choice
Which of the following statements regarding 454 pyrosequencing is not true?
A) Each DNA fragment to be sequenced must be clonally amplified.
B) Dideoxy nucleotides are used to reversibly terminate chain elongation.
C) Nucleotide incorporation is detected as light generated by the luciferase enzyme.
D) Many sequencing reactions are carried in in parallel in a multi-well plate.
Correct Answer:

Verified
Correct Answer:
Verified
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