The Sequence of a Portion of a Gene Is Presented

Essay

The sequence of a portion of a gene is presented below:
5′-AGCAATGCATGCATCGTTATGG-3′
3′-TCGTTACGTACGTAGCAATACC-5′
(a) Assuming that transcription starts with the first T in the template strand of the DNA, and continues to the end, what would be the sequence of the transcribed mRNA?
(b) Identify the initiation codon in this mRNA.
(c) Would there be an effect on translation of changing the first C in the template strand to a G? If so, what effect?
(d) Would there be an effect on translation of changing the third G in the template strand to a T? If so, what effect?
(e) Would there be an effect on translation of changing the last A in the template strand to a C? If so, what effect?
(f) Would there be an effect on translation if pyrrolysine were present?