Question 1

In preparing tissue for routine light microscopic study, which procedure immediately precedes clearing the specimen with an organic solvent?

Accepted Answer

A)   Dehydration
B)   Fixation
C)   Staining
D)   Clearing
E)   Embedding A

Question 2

Which of the following staining procedures relies on the cationic and anionic properties of the material to be stained?

Accepted Answer

A)   Enzyme histochemistry
B)   PAS reaction
C)   H & E staining
D)   Immunohistochemistry
E)   Metal impregnation techniques C

Question 3

Adding heavy metal compounds to the fixative and ultrathin sectioning of the embedded tissue with a glass knife are techniques used for which histologic procedure?

Accepted Answer

A)   Scanning electron microscopy
B)   Fluorescent microscopy
C)   Enzyme histochemistry
D)   Confocal microscopy
E)   TEM E

Question 4

Microscopic autoradiography uses radioactivity and can be employed to study what features in a tissue section?

Accepted Answer

A)   The types of enzymes found in various cell locations
B)   Cellular sites where various macromolecules are synthesized
C)   The sequences of mRNA made in the cells
D)   The dimensions of structures within the cells
E)   The locations of genes transcribed for specific mRNA

Question 5

To identify and localize a specific protein within cells or the ECM, one would best use what approach?

Accepted Answer

A)   Autoradiography
B)   Enzyme histochemistry
C)   Immunohistochemistry
D)   TEM
E)   Polarizing microscopy

Question 6

Resolution in electron microscopy greatly exceeds that of light microscopy due to which of the following?

Accepted Answer

A)   The wavelength of the electrons in the microscope beam is shorter than that of a beam of light.
B)   The lenses of an electron microscope are of greatly improved quality.
C)   For electron microscopy the tissue specimen does not require staining.
D)   The electron microscope allows much greater magnification of a projected image than a light microscope provides.
E)   An electron microscope can be much more finely controlled than a light microscope.

Question 7

Cellular storage deposits of glycogen, a free polysaccharide, could best be detected histologically using what procedure?

Accepted Answer

A)   Autoradiography
B)   Electron microscopy
C)   Enzyme histochemistry
D)   H&E staining
E)   PAS reaction

Question 8

Hospital laboratories frequently use unfixed, frozen tissue specimens sectioned with a cryostat for rapid staining, microscopic examination, and diagnosis of pathologic conditions. Besides saving much time by avoiding fixation and procedures required for paraffin embedding, frozen sections retain and allow study of what macromolecules normally lost in the paraffin procedure?

Accepted Answer

A)   Carbohydrates
B)   Small mRNA
C)   Basic proteins
D)   Acidic proteins
E)   Lipids

Question 9

In a light microscope used for histology, resolution and magnification of cells are largely dependent on which component?

Accepted Answer

A)   Condenser
B)   Objective lens
C)   Eyepieces or ocular lenses
D)   Specimen slide
E)   The control for illumination intensity

Question 10

In situ hybridization is a histologic technique used to visualize what type of macromolecule?

Accepted Answer

A)   Proteins
B)   Carbohydrates
C)   Certain enzymes
D)   Nucleic acids
E)   Lipids

Exam 1: Histology Itsmethods of Study