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You Are Performing an Experiment to Study Replication of DNA

Question 22

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You are performing an experiment to study replication of DNA on a specific yeast chromosome. Yeast cells were synchronized in G1 and were released into S phase. DNA was recovered every five minutes after release into S phase, and an analysis was done to look for DNA replication in specific regions of the yeast chromosome. Assume that these regions of the yeast chromosome are adjacent to each other in the following order: Region A, Region B, Region C, Region D. The data below indicate the times during which DNA replication was observed in each of these regions. Using these data, which region or regions of the yeast chromosome most likely contained an origin of replication?
Times When DNA Replication Was Observed in Each Region:
Region A: 40 minutes, 45 minutes, and 50 minutes
Region B: 35 minutes, 40 minutes and 45 minutes
Region C: 30 minutes, 35 minutes, and 40 minutes
Region D: 35 minutes, 40 minutes, and 45 minutes


A) Region A
B) Region B
C) Region C
D) Region D
E) Regions A, B, C and D

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