Each Origin of Replication in Eukaryotic Cells Is Recognized by an Origin

Multiple Choice

Each origin of replication in eukaryotic cells is recognized by an origin recognition complex, which allows the assembly of the pre-replicative complex including the replicative helicase. This complex ultimately allows the recruitment of DNA polymerase. After DNA polymerase is recruited to an origin and replicates the DNA in that region during S phase, the replicative helicase cannot be loaded onto the DNA in that region again until early G1 due to a variety of regulatory mechanisms. Why is it important that, once used, the replicative helicase cannot be loaded onto the DNA again until G1 of the cell cycle?

A) This ensures that multiple origins must be used to replicate the DNA in all cellular chromosomes.
B) This ensures that DNA cannot be replicated again in that region until the next cell cycle.
C) This ensures that telomeres on all chromosomes will be replicated properly.
D) This ensures that DNA repair processes can only take place during G1 of the cell cycle.