If During the Process of DNA Replication,the Enzyme Topoisomerase Was

Multiple Choice

If during the process of DNA replication,the enzyme topoisomerase was not functioning (i.e.it was mutated) what do you expect will happen to the replication process?

A) The double helix would not begin to unwind at all.
B) The double helix would begin to unwind but this unwinding would stop prematurely due to over-twisting of the DNA.
C) The double helix would unwind completely but no primers would be added to the template strand.
D) The double helix would unwind completely but no new strand would be created due to the formation of secondary structures.