You Ask Your Student to Transfer a Plasmid-Free Bacterial Strain

Multiple Choice

You ask your student to transfer a plasmid-free bacterial strain to multiple small tubes for long term storage.A short while later,your student admits that he did not use a clean sterile pipette to transfer the bacteria to the small tubes,but rather grabbed the same pipette he had just used to collect newly transformed bacteria.Why would this pose a problem?

A) Plasmids can be transferred between bacteria via pili.
B) Newly transformed bacteria will actively undergo binary fission.
C) Plasmid can contain genes that confer antibiotic resistance.
D) Plasmids will incorporate into the chromosomal DNA.