Replication Is Initiated at a Replication Origin, Where an Initiator

Short Answer

Replication is initiated at a replication origin, where an initiator protein binds and causes a short stretch of DNA to unwind. DNA helicase breaks hydrogen bonds at a replication fork, and singlestrand-binding proteins stabilize the separated strands. DNA gyrase reduces the torsional strain that develops as the two strands of double-helical DNA unwind.
-Place the following components in the order in which they are first used in the course of replication: helicase, single-strand-binding protein, DNA gyrase, initiator protein.