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Which of the Following Steps Is NOT Involved with Each

Question 16

Multiple Choice

Which of the following steps is NOT involved with each cycle of amplification in PCR?


A) the addition of fresh dNTPs to the reaction mixture
B) annealing of oligodeoxyribonucleotide primers to DNA
C) thermal denaturation of the target duplex DNA
D) reaction with DNA polymerase at approximately 70°C

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