Real-Time PCR Can Be Used to Quantify the Original Amount

Multiple Choice

Real-time PCR can be used to quantify the original amount of RNA in a sample. What additional step must be accomplished in this particular application?

A) the careful design of specific forward and reverse primers
B) RNA must first be converted to cDNA.
C) RNA must be denatured with formaldehyde to avoid secondary structures.
D) the separation of mRNA from other RNA species
E) DNA is converted to RNA.