Multiple Choice
In the Sanger method of DNA sequencing,incorporation of the nucleotides stops because
A) the dideoxynucleotide analogs have no 3'-OH group to continue DNA synthesis.
B) the dideoxynucleotide analogs have no 5'-OH group to continue DNA synthesis.
C) the dideoxynucleotide analogs have no 2'-OH group to continue DNA synthesis.
D) the mixture is heated to 92°C to stop DNA synthesis at specific time intervals.
E) the dideoxynucleotide analogs are in greater concentration than the actual nucleotides.
Correct Answer:
Verified
Correct Answer:
Verified
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