You Expect That a Mutation in E

Multiple Choice

You expect that a mutation in E.coli that was recently isolated results in loss of enzyme R activity.In looking for the gene,you find comparable sequences.Using an in vitro transcription translation system,you use the gene obtained from the mutant,as well as a wild type E.coli as a control.Following protein isolation and gel electrophoresis,you observe that the wild type E.coli produces a protein that is 35K in size,corresponding to enzyme R.However,the mutant strain produces a protein that is 25K in size.What does this information suggest?

A) A nonsense mutation has occurred, resulting in a truncated, nonfunctional product in the mutant.
B) A large addition has occurred in the wild type strain, such that approximately 35 tandem repeats of this gene are present and are biologically active.
C) A missense mutation has occurred, resulting in a truncated, nonfunctional product in the mutant.
D) all of the above
E) Not enough information is given.