In the Amino Acid Analysis the PTC-Amino Acid Derivatives of a Hydrolyzed

Multiple Choice

In the amino acid analysis the PTC-amino acid derivatives of a hydrolyzed protein are subjected to HPLC.How do you determine which amino acids are present?

A) From their elution time from the HPLC column
B) From the value of the absorbance at 254 nm
C) From the value of the absorbance at 257 nm
D) From conductivity of the separated derivatives