Use the Following Information to Answer the Questions Below

Multiple Choice

Use the following information to answer the questions below.
Yeast is capable of synthesizing the amino acids necessary for survival and can grow on minimal media or enriched media easily. In both conditions, the colonies have a cream colour. The production of adenine is regulated by two genes, ADE1 and ADE2. If either of these genes is defective, adenine cannot be synthesized and the colony will look red (as a result of accumulation of precursors) . A common undergraduate lab study is to irradiate yeast cells with ultra violet light from 2-5 sec up to 1-5 minutes, and see what will happen.
-If, after irradiating and growing cultures in enriched media, you see some red colonies, what is the easiest way to confirm that the phenotype observed is due to a lack of adenine metabolism?

A) Grow them with and without adenine in the media.
B) Grow them with and without the adenine precursor.
C) You would have to see the DNA sequences.
D) Look for the protein products and compare them to wild type.
E) See if the colonies could survive with a higher dosage of UV irradiation.