In Phi X 174 Phage, Whose Genome Is a Single-Stranded

Multiple Choice

In phi X 174 phage, whose genome is a single-stranded, circular DNA known as the - strand, replication occurs by the rolling circle method. In which order must the events of replication occur?

A) nicking of the - strand, binding of relaxase protein to the 5′ end, DNA synthesis of a + strand, separation of + from - strands, circularization of both strands
B) synthesis of a + strand, nicking at the origin, binding of protein to the 5′ end, DNA synthesis, displacement of the + strand when replication passes the origin, circularization of the + strand
C) synthesis of a + strand, nicking of both strands to separate them, recircularization of both strands
D) binding of a protein at the origin of the - strand, synthesis of a + strand, release of the + strand