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Question 38

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To map genes of a bacterial strain,conjugation must be interrupted at given times.Suppose you have Hfr cells of genotype a⁺ᵇ⁺ᶜ⁺ᵈ⁺ᵉ⁺ˢᵗʳᴿ and F⁼ cells of genotype a⁻ᵇ⁻ᶜ⁻ᵈ⁻ᵉ⁻ˢᵗʳˢ and you combine these two cultures in liquid medium in four blenders at time 0.After intervals of 3,6,9,and 12 minutes,you turn on successive blenders.
-What role does the blender play in the experiment?


A) serves as a culture container only
B) acts as a mechanism to bring cells into contact
C) speeds up molecular movement of DNA
D) shears the sex pili of conjugants
E) hastens conjugation events

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