We Can Detect the SNP in the βˢ Allele Because

Essay

We can detect the SNP in the βˢ allele because it destroys a DdeI restriction site,leading to DNA restriction fragment variation revealed by Southern blot analysis.
Either two or three restriction sequences for the restriction endonuclease DdeI can occur near the β-globin gene,depending on the allele.One DdeI restriction sequence is eliminated from the βˢ allele by the SNP in the mutant allele because of a mutation in the double-stranded target sequence 5′ - CTNAG - 3′.
Suppose the wild-type allele sequence is CCTGAG,while a newly identified SNP is CCTTAG.Would this same restriction fragment analysis technique be appropriate for detecting this particular SNP? What other technique(s)could you use to identify this particular mutation?