In Reverse Genetics, What Is the Correct Order in Which

Multiple Choice

In reverse genetics, what is the correct order in which the experimenter proceeds?

A) silencing the genes in question using RNAi, followed by generating gain-of-function alleles
B) screening genes via PCR, followed by deletion of the flanking sequences
C) selection of a phenotypic alternative for the gene in question and sequencing its DNA
D) induction of and selection for a mutant allele with a known sequence, followed by screening for mutations of interest
E) random bombardment of the DNA with a known mutagen, followed by observation of offspring for newly acquired traits