Bacterial Cells with Thick Peptidoglycan and Numerous Teichoic Acid Cross-Linkages

Multiple Choice

Bacterial cells with thick peptidoglycan and numerous teichoic acid cross-linkages are Gram stained.The organisms stain pink.Which one of the following statements explains this discrepancy?

A) The bacterial cells were most likely not heat-fixed to the slide.
B) The bacterial cells were subjected to the decolorizer too long and were overdecolorized.
C) The lot of Gram-stain reagent is most likely expired.
D) No discrepancy exists;cells with this composition stain pink on Gram stain.