You Are Observing the Rate of an Enzymatic Reaction

Multiple Choice

You are observing the rate of an enzymatic reaction.You continue to add substrate in higher and higher concentrations,but eventually you see the rate of reaction plateau,no further increases in the rate of the reaction could be observed.Which of the following are plausible explanations for your observation (select all that apply) ?

A) The enzyme has become saturated.
B) The substrate solution has become contaminated with an allosteric inhibitor.
C) The enzyme molecules are being used up and broken down by the reaction.
D) Over time,the substrate molecules are denaturing.
E) The temperature of the room needs to increase to increase the rate of reaction.