Question 1

You have isolated what appears to be alien DNA. While studying its replication, you performed the exact experiment Meselson and Stahl did. After three generations, the DNA is subjected to a CsCl gradient and only one band appears. What type of replication does this DNA undergo?

Accepted Answer

A) Semiconservative 
B) Conservative 
C) Dispersive C

Question 2

DNA polymerase III has an error in replication once every 100 million nucleotides.

Accepted Answer

A) True 
 B)False  True

Question 3

Which of the following best describes the mechanism of DNA replication in which both a parental strand and daughter strand are combined following replication?

Accepted Answer

A) Dispersive 
B) Semi-conservative 
C) Conservative 
D) All of the answers are correct B

Question 4

Bacterial DNA has how many origins of replication?

Accepted Answer

A) 0 
B) 1 
C) 10 
D) Depends on the size of the DNA

Question 5

You have discovered a strain of

Accepted Answer

A) $\alpha$ 
B) $\beta$ 
C) $\varepsilon$ 
D) $\sigma$ 
E) coli that grows very slowly-the generation time is nearly 12 hours compared to the normal 20-30 minutes.Upon further investigation, you find a mutation in the DNA polymerase III gene.What subunit of the holoenzyme does this mutation affect the most?

Question 6

DNA polymerase III is the main replication enzyme in prokaryotes. What is it in eukaryotes?

Accepted Answer

A) DNA pol a 
B) DNA pol d 
C) DNA pol b 
D) DNA pol g

Question 7

Which of the following is a restriction placed on DNA polymerase?

Accepted Answer

A)  DNA polymerase can attach new nucleotides only in the 5' to 3' direction. 
B)  DNA polymerases must begin synthesis using an RNA primer. 
C)  DNA polymerases must have a template strand to copy from. 
D)  All of these are restrictions of DNA polymerase.

Question 8

The ability of the DNA polymerase to remove mismatched bases is called exonuclease cleavage (proofreading).

Accepted Answer

A) True 
 B)False

Question 9

DNA polymerase is a primer-dependent enzyme that functions only in the 5'-3' direction. These are the two most fundamental concepts to understanding this enzyme. Based on this, which of the following enzyme pairs are analogous in prokaryotes and eukaryotes?

Accepted Answer

A) DNA pol I : DNA pol $\alpha$ 
B) DNA pol II : DNA pol $\beta$ 
C) DNA pol III : DNA pol $\sigma$ 
D) All of the answers are correct

Question 10

The movement of the replication fork in bacterial replication is unidirectional.

Accepted Answer

A) True 
 B)False

Question 11

Catenanes can only form in cells with circular chromosomes, such as bacteria.

Accepted Answer

A) True 
 B)False

Question 12

DNA polymerase III can synthesize a new strand of DNA at the rate of 300 nucleotides per minute.

Accepted Answer

A) True 
 B)False

Question 13

Fills in small regions of DNA where the RNA primers were located.

Accepted Answer

A) DNA ligase 
B) DNA primase 
C) Topoisomerase 
D) DNA polymerase I 
E) DNA polymerase III

Question 14

Which of the following best describes the mechanism of DNA replication in which both parental strands remain together following replication?

Accepted Answer

A) Dispersive 
B) Semi-conservative 
C) Conservative 
D) All of the answers are correct

Question 15

Which of the following is not correct concerning the initiation of bacterial replication?

Accepted Answer

A) It involves a region of the DNA called oriC 
B) DNA proteins bind to the DNA to begin separation of the strands 
C) The strands are initially separated at GC-rich regions of DNA 
D) Following initial separation, DNA helicase enzymes continue to unwrap the DNA

Question 16

Which of the following is an example of a processive enzyme?

Accepted Answer

A) DNA polymerase I 
B) DNA polymerase III 
C) DNA ligase 
D) Okazaki fragments

Question 17

Synthesizes the lagging strand of the DNA.

Accepted Answer

A) DNA ligase 
B) DNA primase 
C) Topoisomerase 
D) DNA polymerase I 
E) DNA polymerase III

Question 18

What functions are accomplished by the primosome?

Accepted Answer

A) Tracking along DNA 
B) Tracking along DNA, separating double stranded DNA 
C) Tracking along DNA, separating double stranded DNA, synthesizing RNA primers 
D) Tracking along DNA, separating double stranded DNA, synthesizing RNA primers, adding nucleotides

Question 19

DNA polymerase III has the ability to begin synthesis of the new daughter strands immediately following the formation of the replication fork.

Accepted Answer

A) True 
 B)False

Question 20

Which of the following best describes the double-helix of DNA?

Accepted Answer

A) It has directionality 
B) The strands are arranged in an anti-parallel arrangement 
C) The strands are complementary 
D) All of the answers are correct

Exam 11: DNA Replication

You have isolated what appears to be alien DNA. While studying its replication, you performed the exact experiment Meselson and Stahl did. After three generations, the DNA is subjected to a CsCl gradient and only one band appears. What type of replication does this DNA undergo?

DNA polymerase III has an error in replication once every 100 million nucleotides.

Which of the following best describes the mechanism of DNA replication in which both a parental strand and daughter strand are combined following replication?

Bacterial DNA has how many origins of replication?

You have discovered a strain of

DNA polymerase III is the main replication enzyme in prokaryotes. What is it in eukaryotes?

Which of the following is a restriction placed on DNA polymerase?

The ability of the DNA polymerase to remove mismatched bases is called exonuclease cleavage (proofreading).

DNA polymerase is a primer-dependent enzyme that functions only in the 5'-3' direction. These are the two most fundamental concepts to understanding this enzyme. Based on this, which of the following enzyme pairs are analogous in prokaryotes and eukaryotes?

The movement of the replication fork in bacterial replication is unidirectional.

Catenanes can only form in cells with circular chromosomes, such as bacteria.

DNA polymerase III can synthesize a new strand of DNA at the rate of 300 nucleotides per minute.

Fills in small regions of DNA where the RNA primers were located.

Which of the following best describes the mechanism of DNA replication in which both parental strands remain together following replication?

Which of the following is not correct concerning the initiation of bacterial replication?

Which of the following is an example of a processive enzyme?

Synthesizes the lagging strand of the DNA.

What functions are accomplished by the primosome?

DNA polymerase III has the ability to begin synthesis of the new daughter strands immediately following the formation of the replication fork.

Which of the following best describes the double-helix of DNA?