You Have Made a Specific DNA Probe That Will Bind

Multiple Choice

You have made a specific DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae, allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens.However, upon testing it out against a known Neisseria gonorrhoeae culture, you find that it does not work.Which of the following is a possible explanation for this negative result?

A) You forgot to label the probe sequence with a reporter molecule.
B) You forgot to add the mRNA to the DNA probe sample.
C) You forgot to digest the probe with restriction endonucleases.
D) You forgot to incubate the test at the 75^oC temperature required for hybridization.