When Studying a New Protein,it Is Useful to Generate an Antibody

Multiple Choice

When studying a new protein,it is useful to generate an antibody against it to tag it in experiments designed to study its function and localization.Which of the following is NOT a step needed to generate this new antibody?

A) Mouse spleen cells are fused with myeloma cells.
B) Cells are selected for their ability to divide and grow in the absence of purines in the medium.
C) Mice are injected with the antibody that will tag the new protein.
D) The spleen is isolated from a laboratory mouse.